Sequence studies of nonradioactive Mycoplasma tRNAPhewith the aid of polynucleotide phosphorylase and polynucleotide kinase
نویسندگان
چکیده
منابع مشابه
Studies on Polynucleotides Synthesized by Polynucleotide Phosphorylase
The preparation of synthetic ribonucleic acid in which one of the nucleotide species is labeled with P32, makes possible studies on the distribution of the labeled nucleotide in the polynucleotide chain. Fig. 1 presents the structure of a polynucleotide in which labeled adenylic acid is randomly distributed. Hydrolysis of such a polymer with snake venom phosphodiesterase (Fig. 1A) will yield a ...
متن کاملPolynucleotide phosphorylase of Micrococcus lysodeiktpcus. III. The apparent arsenolysis of nucleoside diphosphates by polynucleotide phosphorylase.
The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
متن کاملThe origin of polynucleotide phosphorylase domains.
In this report, we document the presence of polynucleotide phosphorylase (PNPase) in the animal eukaryotes. These proteins contain several domains, including 2 RNase PH domains (PNPase 1 and PNPase 2) which are closely related functionally and in sequence similarity to ribonuclease PH (RPH) protein. Phylogenetic analysis of the gene genealogy of these three domains suggests that PNPase was form...
متن کاملThe mechanism of action of polynucleotide phosphorylase.
The purpose of this paper is to review certain aspects of the mechanism of action of polynucleotide phosphorylase and to present, rather briefly, some recent findings. The discussion will be concerned with studies carried out by S. Ochoa and his associates a t New York University, New York, N. Y., and with work done a t the National Institutes of Health. Reference will also be made to some rece...
متن کاملFrom polynucleotide phosphorylase to neurobiology.
In the fall of 1944, I enrolled at the Hebrew University of Jerusalem. The student body numbered about 700 and the choice of faculties was somewhat limited.My hopewas to study medicine, but the plans to open a medical school were still at the drawing board stage. I therefore chose to study chemistry with biochemistry and bacteriology as minor subjects, which I thought I would need later if I we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1974
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/1.12.1733